Fever is one of the most common clinical symptoms of many diseases. Qingkailing (QKL) injection is widely used in China as a\r\nclinical emergencymedicine due to its good antipyretic effects. It is a herbal formula which is composed by eight kinds of traditional\r\nChinese medicines (TCM). As a kind of typical multiple constituents and multiple actions of TCM, it is very difficult to elaborate\r\nthe antipyretic mechanism by conventional pharmacological method. Metabonomics technique provides beneficial tool for this\r\nchallenge. In this study, an ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC QTOF/\r\nMS) metabonomics method was developed to explore the changing process of biochemical substances in rats of yeast-induced\r\npyrexia. Partial least squares discriminate analysis (PLS-DA) was used to distinguish the normal control group, the pyrexia model\r\ngroup, and the pyrexia model group treated by QKL injection.The potential biomarkers related to pyrexia were confirmed and\r\nidentified.MetPA was used to find the possible metabolic pathways.Theresults indicated that the antipyretic effect ofQKL injection\r\non yeast-induced pyrexia rats was performed by repairing the perturbed metabolism of amino acids.
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